贵州稻瘟病菌群体的遗传多样性初步研究

On diversity of population genetics of Magnaporthe grisea in Guizhou

采用Rep-PCR分子指纹技术对2003-2005年采自贵州省内的稻瘟病菌Magnaporthe grisea菌株进行DNA指纹分析,结果显示,200个供试菌株分别扩增到2-15条DNA带,经UPGMA聚类分析,在0.774遗传相似水平下,供试菌株划分为19个遗传谱系,141个单元型。优势谱系为GZL7和GZL6,分别拥有49个和26个单元型,其菌株分别占42%和20.5%。研究显示,贵州稻瘟病菌的群体结构呈现多样性和复杂性,同时揭示菌株的遗传谱系与品种和生理环境有明显的相关性。

DNA fingerprinting of Magnaporthe grisea isolates collected from Guizhou was determined with the rep -PCR technique. 2 -15 DNA bands were amplified for each isolate. 200 isolates were classified into 19 genetic lineages and composed of 141 haplotypes at a genetic similarity of 0. 774 in clustering analysis of UPGMA. Of these lineages, GZL7 and GZL6 were dominate lineages with 49 and 26 haplotypes,corresponding to 42% and 20. 5% respectively. The results indicated that genetic structure of M. grisea population in Guizhou province tended to be multiplicity and complexity ,and also revealed that genetic lineage isolates were directly related to variety and environment physiology.