mMCP-1对病毒性心肌炎小鼠心肌病变的干预作用

Mutated monocyte chemoattractant protein - 1 （ mMCP - 1 ） interferes with the cardiac lesion of viral myocarditis

目的研究N端2—8位氨基酸缺失的突变型MCP-1（mutated MCP-1，mMCP-1）对病毒性心肌炎小鼠心肌病变的干预作用。方法CVB3腹腔注射BALB／c小鼠，随机分组：病毒性心肌炎组（A组）、mMCP-1干预组（B组）、空质粒pVAX1对照组（C组）和未感染生理盐水对照组（D组）。比较各组小鼠心脏重量和体重的比值（HW／BW）、心肌组织病理学积分（PS）、心肌组织病毒载量、血清CK—MB水平和心肌浸润的单个核细胞变化。结果与A组相比，B组血清CK—MB水平降低（P〈0．01）、PS减少（P〈0．05），HW／BW和CVB3载量未呈明显改变（P〉0．05）；B组小鼠心肌浸润的单个核细胞减少（P〈0．01），平均下降41、7％；与D组相比，B组CK—MB、PS、HW／BW和CVB3载量均明显升高。而与A组相比，C组CVB3载量、血清CK—MB水平、HW／BW和心肌组织病理学积分均未呈明显改变。结论mMCP-1可缓解病毒性心肌炎中心肌组织病变和心肌损伤，这与单个核细胞浸润减少有关。

Objective To investigate the intervention of monocyte chemoattractant protein - 1 （ MCP - 1 ） deleted the 2 - 8 amino acid in N - terminus（ mutated MCP - 1, mMCP - 1 ） with the cardiac lesion in viral myocarditis induced by coxsackievirus group B type 3 （CVB3）. Methods BALB/c mice inoculated intraperitoneally with CVB3 were divided into four groups including viral myocarditis group infected alone with CVB3 （group A） ,intervention group with mMCP- 1 （goup B）, control group with plasma pVAX （group C）, and the normal control group （group D） inoculated intraperitoneally with saline was designed. Ratio of mouse heart weight to body weight （HW/BW）, myocardial tissue pathological score （PS） of cardiac tissue ,the level of CK- MB in serum and CVB3 loading of myocardial tissue were analyzed. Results Compared with group A,the level of creatine kinase -MB fraction （CK -MB） in group B decreased （ P 〈0.01） and PS also reduced （ P 〈0.05） ,but HW/BW and CVB3 loading in myocardial tissue did not changed greatly （ P 〉 0.05 ）. And there was much difference between group B and group D in the level of CK - MB, PS and CVB3 loading of myocardial tissue. Compared with Group A,group C＇s CVB3 loading in myocardial tissue,CK - MB level in serum,HW/BW and PS all did not alter markedly （ P 〉 0.05）. Conclusion mMCP - 1 could alleviate the cardiac lesion and cardiac injury of viral myocarditis,which is associated with improvement of the infiltration of mononuclear cells.