ESAT6抗原刺激产生INFγ水平测定在HIV感染者中对结核病诊断的价值

Tuberculosis diagnostic value of the INFγ level by activation of ESAT6 antigen in HIV infector

目的探讨ESAT6抗原刺激后测定γ干扰素（INFγ）在HIV感染者中对结核病的诊断价值。方法同时抽取HIV感染者胸部正常、涂阴肺结核、涂阳肺结核病人的全血，于24h内检查，以加入植物血凝素（PHA）为对照组，同时以加入ESAT6抗原为研究组，培育后测定全血中INFγ水平，以ESAT6抗原组较对照组INFγ增加的百分比为评价指标。结果以INFγ增加≥1％、5％、10％、15％为阳性分割值，活动性肺结核病人检出的敏感度分别为76％、67％、60％、54％，特异度分别为67％、77％、80％、80％。结论ESAT6抗原刺激后测定INFγ在HIV／AIDS病人中对结核病的诊断价值较大。

Objective To observe the tuberculosis diagnostic value of the INFγ level by activation of ESAT6 antigen in HIV infector. Methods Taking out whole blood of the persons with chest in gear, smear negative tuberculosis and smear positive tuberculosis in HIV infector. The blood which was swing in with PHA was control group in 24 hours, the blood which was swing in with ESAT6 antigen was investigation group in 24 hours. The indicate is the increased percent of INFγ of the investigation group than the control group. Results The positive value of INFγ increased percent was ≥1% ,5% ,10%, 15% ,respectively. Then the sensitivity of activity tuberculosis detection was 76% ,67% ,60% ,54% respectively. The speciality of the persons with chest in gear was 67% ,77% ,80% ,80% respectively. Conclusion The tuberculosis diagnostic value of the INFγ level by activation of ESAT6 antigen in HIV infector is bigger .