MDM2与P53在宫颈鳞癌中的表达及相关性研究

Expression of MDM2 and P53 in the squamous carcinoma of the cervix and its relationships

目的探讨MDM2与P53蛋白在宫颈鳞癌中的表达及相关性，进一步探讨宫颈鳞癌的发生和发展机制。方法选取2003年3月至2006年3月郑州大学第三附属医院50例宫颈鳞癌手术切除标本，50例正常宫颈黏膜组织，30例癌旁非典型增生组织。采用免疫组化SP法检测MDM2与P53蛋白的表达情况。结果宫颈鳞癌组织、癌旁非典型增生组织与正常宫颈黏膜组织中MDM2蚤白阳性表达率依次降低，且癌组织、癌旁非典型增生组织及正常宫颈黏膜组织中MDM2蛋白表达间相比，差异有统计学意义（P〈0．05）。宫颈鳞癌组织、癌旁非典型增生组织与正常宫颈黏膜组织中P53蛋白阳性表达率依次降低，癌组织、癌旁非典型增生组织及正常宫颈黏膜组织中P53蛋白表达间相比，差异有统计学意义（P〈0．05）。结论MDM2和P53在宫颈黏膜从良性病变到癌前病变到癌的进展过程中发挥重要作用。

Objective To explore the expression of MDM2 and P53 protein in the squamous carcinoma of the cervix and its relationships, and explore the mechanisms of occurrence and development of the squamous carcinoma of the cervix. Methods To detect the expressions of MDM2 and P53 protein in the 50 cases of squamous cell carcinoma of cervix,30 cases of atypical hyperplasia tissues of tumor - adjacent and 50 cases of normal mucosa of uterine cervix respectively from the third affiliated hospital of Zhengzhou university from march 2003 to march 2006 by immunohistochemistry SP method. Results The positive expression rated of MDM2 protein in the squamous carcinoma of the cervix, atypical hyperplasia tissues of tumor - adjacent normal mucosa of uterine cervix were lower in turn, furthermore the positive expression rate of MDM2 protein in the carcinoma tissues is compared with that in the propotional adjacent tissues and normal mucosa tissues from each other,there is statistical significance（ P 〈 0. 05 ）. The positive expression rates of P53 protein in the squamous carcinoma of the cervix, atypical hyperplasia tissues of tumor - adjacent normal mucosa of uterine cervix were lower in turn, furthermore the positive expression rate of P53 protein in the carcinoma tissues is compared with that in the propotional adjacent tissues and normal mucosa tissues from each other, there is statistical significance（ P 〈 0. 05 ）. Conclusion MDM2 and P53 play the significant roles during the process of benign lesion to lesion precancerous of mucosa of uterine cervix.