异丙酚对大鼠肾缺血再灌注损伤后肾组织ICAM-1表达的影响

Effect of Propofol on Expression of ICAM-1 in Acute Renal Ischemia/ Reperfusion Injury in Rats

目的探讨异丙酚不同给药时机对大鼠肾缺血再灌注损伤后肾组织ICAM-1表达的影响。方法50只SD大鼠随机分为5组,每组10只:假手术对照组(C)、缺血再灌注组(I)、及异丙酚预先给药组(P1),异丙酚即时给药组(P2),异丙酚延迟给药组(P3)。缺血前1h,C、I组经鼠尾静脉以2mL/h速度输注生理盐水,P1组阻断前1h经鼠尾静脉缓注异丙酚30mg/kg/h,继以持续输注30mg/kg/h至松开后3h,P2组阻断肾动脉同时静脉缓注异丙酚30mg/kg/h至松开后4h,P3组在松开阻断同时静脉缓注异丙酚30mg/kg/h至松开后4h 45min。阻断肾动脉后关闭腹腔,缺血45min后,再次打开腹腔,显露肾脏,松开动脉夹,逐层缝合腹部正中切口,再灌注24h处死大鼠。观察血肌酐(Cr)、尿素氮(BUN),肾组织ICAM-1mRNA表达及ICAM-1蛋白的变化。结果I组血Cr、BUN与C组比明显增高(P<0.05),P1和P2组较I组明显下降(P<0.05),P3组与I组相比无统计学意义(P>0.05)。肾缺血再灌注后,肾组织ICAM-1 mRNA和蛋白表达明显增加(P<0.01),P1和P2组与I组相比可明显减少ICAM-1 mRNA及其蛋白表达(P<0.01或<0.05),P3组的变化不明显,无统计学意义(P>0.05)。结论异丙酚预先和即时给药对大鼠肾缺血再灌注损伤有明显的保护作用,此保护部分是通过减少ICAM-1 mRNA及其蛋白表达来实现的,而延迟给药无此保护作用。

Objective This study aimed at assessing the effects of propofol on expression of ICAM-1 in acute renal ischemia/reperfusion injury.Methods Fifty SD rats were randomly divided into five experimental groups.In Group C,after anesthetization,renal artery was not ligated,kidney were exposed for 45 min,then the abdomen was closed in two layers.In Group Ⅰ, renal artery were ligated for 45min,and kidneys were reperfused for 24h, then the rats were killed; In Group P1,Group P2 and Group P3,propofol infusion at 30 mg/kg/h was performed 1h before, simultaneous with or 45min after the renal ischemia in the pretreatment, simultaneous treatment and post treatment groups respectively, propofol infusion at 30 mg/kg/h up to 3h,4h,4h 45min differently.And reperfused for 24h, the rats were killed.Then blood Cr, BUN,ICAM-1 mRNA and ICAM-1 protein of renal were measured.Results BUN and Cr increased strongly in ischemic reperfusion group when compared to sham group（P〈0.05）.BUN and Cr was significantly decreased in Pre and simultaneous treatment group when compared with the sham group （P〈0.05）. The change of BUN and Cr in post-treatment was not marked （P〉0.05）.ICAM-1 mRNA expression and ICAM-1 protein were significantly increased in I/R group compared with control group （P〈0.01）.That in Pre and simultaneous treatment group down-regulated markedly when compared with group Ⅰ （P〈0.01 or P〈0.05）.There was no significant difference between in post-treatment group and group Ⅰ （P〉0.05）.Conclusion Pre and simultaneous treatment with propofol is effective in protecting the renal injury from renal ischemia/reperfusion by reducing the ICAM-1 mRNA and ICAM-1 protein expression.