内切葡聚糖酶基因工程菌pET-C36表达条件的研究

Research on Expression Conditions of Endoglucanase in the Recombinant E. coli Strain

为了提高内切葡聚糖酶基因在大肠杆菌中的表达量,通过实验研究了重组菌Pet-C36的表达条件,结果表明重组菌的培养条件为:最适培养温度43℃,最适培养时间5 h,IPTG诱导终浓度0.1 mmol/L或乳糖诱导终浓度0.1%。基因工程菌在此条件下诱导培养后酶活力比未优化表达条件时提高了2倍,可达141.22 U/mL。

In order to improve the expression level of endoglucanase gene in E. coli, the expression conditions of the recombinant were studied in this experiment. The results showed that： the recombinant E. coli was incubated at 43 ℃ for 5 h, and when induced by 0.1 mmol/L IPTG or 0.1% lactose, its yield of endoglucanase can come to 141.22 U/mL which is about 2 times higher than that of the control.