三七茎叶提取物指纹图谱的建立

Construction of Chromatographic Fingerprint of Saponins in Panax Notoginseng Leaves

利用高效液相色谱-紫外检测技术(HPLC-UV)对三七茎叶提取物进行分离,摸索出适宜的分离条件。当用乙腈-水(体积比为32∶68)流动相体系等度洗脱,流速0.7 mL/min,检测波长203 nm时,三七茎叶提取物中各组分得到了较好的分离。本试验方法具有很高的精密性,稳定性和重现性。为了进一步研究三七茎叶提取物的指纹图谱,对10批三七茎叶提取物样品在相同的色谱条件下进行分析。结果表明,在三七茎叶提取物中确定了14个“共有峰”,可作为其指纹图谱。此外,利用高效液相色谱-质谱联用技术(HPLC-MS),并对照部分皂甙的标准品,对已经确定的14个“共有峰”定性。三七茎叶提取物指纹图谱的建立有助于今后对其质量进行检测。

At present, the construction of chromatographic fingerprints plays a significant role in the quality control of traditional medicines. In this paper, high performance liquid chromatography-ultraviolet absorption detector（HPLC-UV） profiles of saponins in panax notoginseng leaves were established. The analysis was carried out on a reversed-phase column （150 mm × 4.6 mm,I. D. C18, 5 μm, ） from Agilent maintained at 40 ℃. Separation was performed by elution using acetonitrile and water at a volume ratio of 32 ： 68. The flow rate was 0.7 mL/min. The detection wavelength was set at 203 nm. Under the above condition, peaks were well separated. To develop the representative fingerprint of saponins in panax notoginseng leaves, 10 batches of samples were analyzed under the same HPLC conditions. The results showed that 10 batches of saponins in panax notoginseng leaves have 14 common peaks as their fingerprint. Besides, these common peaks were all characterized by using HPLC-MS technique and comparison with the reference standards. The fingerprint established will help to control the quality of saponins in panax notoginseng leaves.