摘要
目的建立妇科白带片中橙皮苷的含量测定方法。方法色谱柱:Diamond C18(4.6mm×200mm,5μm),流动相:乙睛-0.1%磷酸溶液(21:79),流速1.0ml/min,检测波长283nm。结果橙皮苷在0.0668~0.6012μg范围内呈现良好的线性关系,r=0.9994,平均回收率为96.96%,RSD为1.01%。结论本方法简便、快速、准确,分离效果好。
Objective To establish a simple sensitive HPLC method for determining hesperidin in Puke baibai tables. Method Hesperidin was separated on Diamond C18(4.6mm × 200mm, 5 μm) with a mobile phase of acetonitrile-0.1% phosphoric acid (21:79) and detected at 283nm with a flow rate of 1.0ml/min. Result The linear range was 0.0668- 0.6012 μ g,r=0.9994. The average recovery was 96.96%,RSD=1.01%.Conclusion The method is simple, rapid and reliable, good sperating degree.
出处
《国际医药卫生导报》
2007年第8期74-75,共2页
International Medicine and Health Guidance News