高效液相色谱法测定妇科白带片中橙皮苷的含量

Determination of Hesperidin in Fuke baiabi Tables by HPLC

目的建立妇科白带片中橙皮苷的含量测定方法。方法色谱柱：Diamond C18（4．6mm×200mm，5μm），流动相：乙睛-0．1％磷酸溶液（21：79），流速1．0ml／min，检测波长283nm。结果橙皮苷在0．0668～0．6012μg范围内呈现良好的线性关系，r=0．9994，平均回收率为96．96％，RSD为1．01％。结论本方法简便、快速、准确，分离效果好。

Objective To establish a simple sensitive HPLC method for determining hesperidin in Puke baibai tables. Method Hesperidin was separated on Diamond C18（4.6mm × 200mm, 5 μm） with a mobile phase of acetonitrile-0.1% phosphoric acid （21：79） and detected at 283nm with a flow rate of 1.0ml/min. Result The linear range was 0.0668- 0.6012 μ g,r=0.9994. The average recovery was 96.96%,RSD=1.01%.Conclusion The method is simple, rapid and reliable, good sperating degree.