牛血清白蛋白在修饰氯离子金纳米通道中的迁移研究

Transport Properties of Bovine Serum Albumin in Gold Nanotubule Membrane Modified with Cl-

采用化学镀的方法在聚碳酸酯模板上沉积金,制成金纳米通道膜,并对它进行Cl^-修饰得到带负电荷的通道。再采用电化学方法（i-t法）对牛血清白蛋白（BSA）在修饰通道中的迁移进行研究。在pH7.4PBS中,对通道两端施加1.0V电压,溶液中离子迁移通过纳米通道时产生电流响应。当溶液中加入BSA后,电流响应减小,且响应变化量与浓度在1.50×10^-10-1.35×10^-9mol/L范围内呈线性关系,其线性回归方程为｜Δi｜（μA）=0.0069＋0.125c（×10^-9mol/L）,相关系数为0.9980,检出限为9.46×10^-11mol/L（S/N=3）。

Gold nanotubule membranes were prepared by using electroless deposition of gold within the pores and surfaces of polycarbonate track-etched membranes, and the membranes were modified with Cl^-. Then an electrochemical method （i-t） has been developed for studying the transport properties of bovine serum albumin （BSA） in these nanotubule membranes. In phosphate buffer solution （ PBS, pH 7.4）, when an electric field （1 V） was applied between the two sides of the membrane, the response current decreased with the adding of BSA. The decrement of response current was in linear relationship with concentration of BSA in the range of 1.50×10^-10-1.35×10^-9mol/L, the correlation coefficient was 0. 9980, and the limit of detection was 9.46×10^-11mol/L（S/N=3）.