太子参中环肽PseudostellarinB含量HPCE测定及其指纹图谱研究

Determination of Pseudostellarin B in Pseudostellaria heterophylla by HPCE and Study on Its Fingerprints Analysis

建立了测定太子参中环肽Pseudostellarin B含量的高效毛细管电泳（HPCE）分析方法，对10种不同产地太子参中环肽Pseudostellarin B的含量进行了测定。HPCE工作条件：采用未涂层熔融石英毛细管（内径75μm，有效长度50cm），分离电压为15kV，柱温20℃，二极管阵列检测器（DAD）检测波长为203nm，缓冲液为20mmol／L．硼砂（pH=9．3）溶液。在选定的工作条件下，环肽Pseudostellarin B浓度与其响应信号值之间具有较好的线性相关性（Y=0．6357X＋2．546，R=0．9985），加标回收率在93．8％～105．6％之间。在此基础上进行了太子参HPCE指纹图谱研究，采用中药指纹图谱相似度计算软件对不同太子参样品的HPCE指纹图谱进行相似度计算，以系统生成的对照指纹图谱为对照模板，10份样品中有8份的相似度在0．90以上，说明该方法可用于太子参质量控制。

A modem HPCE analysis method was developed for determinating cyclic peptide Pseudostellarin B in Pseudostellaria heterophylla（ Miq. ） Pax. Separation and determination were carried out by HPCE under the following conditions ： bared fused silica capillary （ 50 cm × 75 μm i. d. ） ,20 mmol/L borate （ pH = 9.3 ） as buffer,the tun voltage is ＋ 15 kV, detection length of UV at 203 nm, and column temperature of 20 ℃. The results indicated that the developed method was simple, accurate and reliable for the determination of Pseudostellarin B with a good linearlty（ Y = 0. 6357X ＋ 2. 546 ,R = 0. 9985 ）, and the recovery ranges of 93.8% ～ 105.6%. Based on this method, the HPCE fingerprints of Pseudostellaria heterophylla （ Miq. ） Pax were established. The fingerprints were compared by the software of the similarity evaluation system for chromatographic fingerprint. The fingerprint congruence coefficients of 8 electropherograms in 10 were above 0.90. The method of HPCE fingerprint is reliable and accurate.