摘要
目的:研究腺病毒介导缺氧诱导因子-1α基因转染对心肌细胞缺血再灌注损伤的保护作用。方法:采用纯化培养的新生大鼠心肌细胞建立缺血再灌注损伤模型,实验分6组:正常细胞培养组,缺氧复氧损伤模型组(I/R组),基因转染小、大剂量组(AdHIF-1α组,MOI50,100),转染腺病毒空载体组(AdBlank组),转染HIF-1α核酶基因组(AtHIF-1α组)。测定各组缺氧复氧后细胞损伤指标乳酸脱氢酶(LDH)、丙二醛(MDA)含量及细胞活性情况。结果:AdHIF-1α组(MOI50,100)较I/R组、AdBlank组、AtHIF-1α组LDH、MDA明显降低,细胞活性高(P<0.05)。结论:腺病毒介导HIF-1α基因转染心肌细胞具有抗缺血再灌注损伤、保护心肌的作用。
Objective：To study the protective effect of adenovirus-mediated HIF-1α gene therapy against ischemia-reperfusion （I/R） injury on cultured rat myocardiocytes. Methods：The models of cultural hypoxia neonatal rat myocardiocytes were divided into six groups： control group, ischemia/reperfusion （I/R） group, two groups transfected with high and low HIF-1α gene dosage （Ad HIF- 1α group, MOI 50,100）, adenovirus vectors group （Ad blank group） and HIF-1α rebozyme gene group （At HIF-1α group）. The levels of lactate dehydrogenase （LDH） in the medium, malondialdehyde （MDA） in myocardiocytes and cell activity were tested. Results： In comparison with I/R group, Ad Blank group and At HIF-1α group,the levels of LDH and MDA in Ad HIF-1α group were significantly decreased （P〈0.05）, together with higher cell activity （P〈0.05）. Conclusion： Adenovirus-mediated transfection of HIF-1α gene can protect cultured myocardiocytes against ischemiareperfusion injury.
出处
《国际心血管病杂志》
2007年第2期137-140,共4页
International Journal of Cardiovascular Disease
