HPLC法测定陕西女贞子中齐墩果酸与熊果酸的含量

Determination of Oleanolic Acid and Ursolic Acid in the Fruits of Ligustrum Iucidum Ait.of Shanxi by HPLC

目的测定陕西境内不同产地、不同生长期女贞子中齐墩果酸与熊果酸的含量。方法采收陕西境内关中3市(渭南、西安、宝鸡)与陕南2市(安康、汉中)10月产女贞子;并采收西安8、9、10、11和12月产女贞子,去杂质阴干,室温密闭贮藏。采用HPLC法,Lichrospher C_(18)(4.6 mm×250 mm,5μm)色谱柱,Waters600高效液相色谱仪,流动相为乙腈-甲醇-水-磷酸-三乙胺(50:30:20:0.02:0.04),流速1mL/min。建立标准工作曲线,检测各地、各时间点样品中齐墩果酸和熊果酸含量。结果安康产女贞子中齐墩果酸与熊果酸含量均达到同期最高。不同生长期女贞子在10月份出现齐墩果酸含量最高值;而熊果酸在幼果中(8月)含量最高并随生长期延长逐渐下降。到12月时二者含量均降至最低。结论女贞子在不同产地、不同生长期齐墩果酸和熊果酸的含量是不同的,故在实际应用时应根据不同需要适时采收。

Objective To determine oleanolic acid （OA） and ursolic acid （UA） from Fructus Ligustri Lucidi in diffremt habitat and various grovring stages. Metheds We picked the fruits of Fructus Ligustri Lucidi in Otober in five cities of Shanxi, and in August, September, October, November and December in Xi＇an. Removed impurities and storaged the fruits under room temperature. By HPLC with Waters 600 as its chromatographic system, and Lichrospher C18 （4.6 mm×250 mm, 5 μm） column was applied with CH3CN-CH3OH-H20-H3PO4-（C2H5）3N （50 ： 30 ： 20 ： 0.02 ： 0.04） as its mobile phase, the flow rate was I mL/min. The standard working curve was made to determine the contents of OA and UA at different habitat and different time spot from samples, Results The contents of OA and UA were highest in Ankang city. During prolonging growing stages, the highest contents of OA and UA were October and August, respectively. They both reduced to the lowest point in December. Conclusions The contents of OA and UA changed in different habitat and diffremt growing stages of Fructus Ligustri Lucidi. It was suggested that we should mainly base on the highest contents to select the harvest time according to our demands.