HPLC法测定梅花点舌丹中华蟾酥毒基和脂蟾毒配基的含量

HPLC Determination of Paeoniflorin in Huachanahuduji and Zhichadupeiji of Meihuadianshe Den

目的建立梅花点舌丹中药材蟾酥中华蟾酥毒基和脂蟾毒配基含量测定方法。方法采用HPLC法,色谱柱为Hypersil ODS(250nm×4.6nm);流动相:乙腈-水(43.5:56.5);柱温:室温;流速:1.0mL/min;检测波长为296nm;进样量20μL.结果华蟾酥毒基和脂蟾毒配基在5.31～63.75μg/mL、6.00～72.00μg/mL范围内与峰面积积分值呈现良好线性关系,相关系数r分别为0.9998和0.9997,平均回收率分别为99.45%、99.39%,RSD分别为1.32%、0.55%(n=6)。结论该方法准确、重复性好,可有效控制该产品的质量。

Objective To establish HPLC method for determination of Huachanshuduji and Zhichadupeiji in Chansu of Meihuadiansheoan. Metheds HPLC system includes ODS column, 4.6mm× 250 mm, the mobile phase was acetonitrile-water （43.5 ： 56.5）, the flow rate was 1.0 mL/min, and detection wavelength was 296 nm. The amount of injection was 20 μL Results the calibration curves of Huachanshuduji and Zhichadupeiji were linear in the range of 5.31～63.75 μg/mL and 6.00～72.00μg/mL （r=0.9998, 0.9997）. The average recoveries concentration added in samples were 99.45%, 99.39%. The deviation was 1.32%, 0.55% （n =6）. Conclusions The method is rapid, accurate and reliable.