油茶物种过氧化物酶与炭疽病抗性

A Study on the Peroxidase in Camellia Species and Their Resistance to Anthracnoso Causing Fungus

以五个对炭疽病抗感性各异的油茶物种为材料。检测和观察了油茶物种受该炭疽菌(Colletotrichum camelliae Massee)感染前后叶和果内过氧化物酶的变动及其原因。结果表明,在供试的五个油茶物种中,以攸县油茶抗病性最高,普通油茶感病性最高,华南油茶、广宁红花油茶和小叶油茶居于这两者之间。在接种的48h以内,过氧化物酶的活性变化值大小与油茶物种的抗病性呈正相关,而在接种的48h以后,过氧化物酶的活性变化值大小与油茶物种的感病性呈正相关。发病前后,抗感性各异的油茶物种过氧化物酶同工酶谱带发生明显变化,感病物种的谱带增加数多于抗病物种。探讨了利用抗性生化指标用于抗病育种的可能性。

Five species of Camellia were used to study the physiological biochemistry of resistance to Colletotrionum camelliae Massee. Test results revealed that Camellia yunsiensis was most resistant, C. neitnamensis was resistant, C. semisererata was moderate resistant, C. meiocarpa was susceptible, C. oleifera was most susceptible. The values of changes in the peroxidase activity were positively correalated with disease resistance of the tested Camellia species within 48h after inoculation, while such values were positively correalated with disease susceptibility of the testad Camellia species over 48h after inoculation. The changes of the peroxidase isoenzyme bands after inoculation were evidently different than before inoculation. There were more bands of changes in the susceptible species than in the resistance ones.