热带假丝酵母辅酶Q_(10)高产菌的选育研究

以热带假丝酵母(Candida tropicalis)AS2.1387为出发菌株,研究紫外线、硫酸二乙酯单因素及复合诱变对出发菌株的诱变效应,并根据辅酶Q10合成的反馈调节途径,建立了高产突变菌株的快速筛选方法,通过放线菌素D对突变菌进行广谱抗性初筛,并以高浓度前体物质对羟基苯甲酸以及终产物结构类似物维生素K3进行特异性筛选,选育得到了遗传稳定性良好的辅酶Q10高产突变株APV.12,使得APV.12菌株辅酶Q10的发酵产量达到23.958mg/L,比出发菌株提高了1.76倍。

Candida tropicalis AS 2.1387 was used as the starting strain and subjected to UV-radiation, DES mutagen or composite mutagenesis. On the basis of feedback regulation mechanism for coenzyme Q10 synthesis pathway, a convenient high productivity coenzyme Q10 mutant screening method was set up. Mutants highly resistant to actinomycin-D were screened out in the first round of screening, and a high productivity coenzyme Q10 mutant APV.12 with good genetic stability the mutants were further identified by specific screening with high - concentration coenzyme Q10 precursor p - hydrobenzoic acid and structure analog of end product vitamin K3, in the second run, and the final coenzyme Q10 yield of the APV.12 reached 23.958mg/L, which was 1.76 times higher than the oriclinal strain.