增强紫外光B辐射对水稻叶片内多胺的影响

Effect of Enhanced Ultraviolet B Radiation on Polyamine in the Leaves of Rice

本试验建立了用内标法检测植物体内苯甲酰化多胺的高效液相色谱法。在以流速为１ｍｌ／ｍｉｎ、流动相为乙腈（５２％ｖ／ｖ）、通过５－μＭＣ１８反相柱在检测峰为２５４ｎｍ的条件下，腐胺、亚精胺、精胺均能完全溶解。由于增加了内标环己二胺，试验的准确度增加。利用高效液相色谱法检测紫外光Ｂ辐射增强对水稻叶片内多胺的影响。结果表明：在２周和４周的处理条件下，紫外光Ｂ（１３．０、１９．１ｋＪ·ｍ－２·ｄａｙ－１）使水稻品种ＩＲ６８和Ｄｕｌａｒ体内的腐胺含量大幅度增加，亚精胺的含量也稍有增加，而精胺含量的变化不明显；紫外光Ｂ促进水稻叶片内多胺的积累与其它逆境因子的作用相似。

A sensitive method has been developed for the analysis of polyamines based on high performance liquid chromatography (HPLC) of their benzoyl dervivatives. Putrescine, spermidine,and spermine can be completely resolved by reversed phase HPLC, isocratic elution with acetonitrile (52%) through a 5 μm C 18 column, and detection at 254 nm. The addition of inter standard hexanediamine resulted in the improvement of precision. The polyamine content in the leaves of rice affected by ultraviolet B (UV B, 280—320 nm) radiation has been detected with HPLC. The results showed that putrescine content in the leaves of rice cultivars IR68 and Dular subjected to UV B radiation (13 0, 19 1 kJ·m -2 ·day -1 ) were significantly increased under two and four weeks treatment. The small increase of spermidine in both cultivars was also observed. However, no remarkable consistent variation in spermine contents affected by UV B in both cultivars was found. Polyamine accumulation in rice subjected to UV B radiation is consistent with similar response to other environment stressors.