氯胺酮对卵蛋白激发的致敏大鼠离体肺泡巨噬细胞胞内钙和自由基的影响

Effect of ketamine on OVA-induced changes of free intracellular calcium and free-radical in sensitized rat macrophage in vitro

目的研究氯胺酮对卵蛋白(OVA)刺激的致敏大鼠肺泡巨噬细胞胞内游离钙离子([Ca2+]i)及自由基的作用,探讨氯胺酮在哮喘治疗中的作用及其机制。方法体外培养的致敏肺泡巨噬细胞经氯胺酮(10、100和1000μM)处理、OVA刺激后,测定细胞[Ca2+]i并检测细胞培养上清液中一氧化氮(NO)和羟自由基(.OH)水平。结果OVA激发可引起致敏肺泡巨噬细胞[Ca2+]i增高及NO和.OH的生成增多,氯胺酮对此具有浓度依赖性抑制作用。结论氯胺酮可抑制OVA所致的氧化应激,其分子机制可能与[Ca2+]i相关。

Objective To observe the effect of ketamine on ovalbumin（OVA）-induced changes of free intracellular calcium （[Ca^2＋] i） and free-radical in sensitized rat macrophages in vitro. Methods Macrophages were collected from bronchoalveolar lavage fluid of OVA-sensitized Sprague Darnley rats,pretreated with ketamine at concentration of 10,100 and 1 000 μM, and then challenged with 100μg/ml OVA. The production of nitric oxide（NO） and hydroxy radical（ ·OH） in the supernatant of macrophages culture was assayed by kits and the level of [Ca^2＋ ]i was measured with fluo- rescent intensity by laser scanning confocal microscope. Results OVA challenge-induced an elevation of [-Ca^2＋] and an increase of NO and ·OH. Ketamine inhibited this response by concentration-related method. Conclusion Ketamine inhibited OVA-induced oxidative stress, in which calcium may be involved.