节律基因Clock对雄性小鼠顶体酶活性的影响

Influence of circadian gene,Clock,on the acrosin activity in male mice

目的通过干扰小鼠睾丸节律基因Clock的表达,研究C ock对雄性小鼠顶体酶活性的影响。方法通过RNAi干扰技术干扰雄性小鼠睾丸节律基因Clock的表达。以Western Blot检测干扰效果。研究干扰Clock表达后对小鼠顶体的形态发育和顶体酶活性的影响。结果CLOCK干扰质粒使小鼠睾丸CLOCK蛋白的表达明显下调,顶体酶活性明显下降。结论节律基因Clock可能通过调节顶体酶活性来影响雄性小鼠的生殖功能。

Objective To study the influence of circadian gene, Clock, on the acrosin activity by attenuating the expression of Clock in male mice. Methods RNAi technology was applied to interfere with the expression of circadian gene, Clock, in testis of mice. The interference efficiency of the iRNAs was evaluated by Western blot. The acrosin activity and the FITC-PSA were measured. Results The RNAi plasmid targeting circadian gene Clock attenuated the expression of Clock in testis of mice, and reduced the acrosin activity of male mice. Conclusion Circadian gene, Clock, influences the male fecundity probably by changing the acrosin activity.