摘要
文章介绍了二甲酚橙法用于测定植物组织中的H2O2,确定了适宜的测定波长、显色反应时间,以及工作试剂中适宜的Fe2+、二甲酚橙、山梨醇浓度和pH值等参数。与其它方法相比,此方法表现出较高的灵敏度、较好的重复性、准确性和可比性。
This paper introduced xylenol orange method used for the measure ment of hydrogen peroxide in olant tissue, the optimal measurement wavelength, reaction time, the contents of Fe^2 + , xylenol orange, sorbitol and the pH value of the working reagent were selected. This method shew high sensitivity and preferable reproducibility, accuracy, and comparability compare with other methods.
出处
《云南师范大学学报(自然科学版)》
2007年第3期50-54,共5页
Journal of Yunnan Normal University:Natural Sciences Edition
基金
云南省自然科学基金(2006C0030M)
教育厅基金(06Y117B)
关键词
二甲酚橙
植物组织
过氧化氢
测定方法
xylenol orange
plant tissue
hydrogen peroxide
measurement method