虎杖苷对小鼠肝细胞色素P450和II相酶的影响

Effects of polydatin on liver cytochrome P450 and phase Ⅱ transferase in mice

目的:研究虎杖苷(polydatin)对肝脏药物代谢酶的影响。方法:小鼠灌服虎杖苷240、120和60mg/kg,给药7天,测定肝微粒体细胞色素P450、b5含量和NADPH细胞色素c还原酶、尿苷二磷酸葡萄糖醛酸转移酶(UDPGT)和谷胱甘肽-S-转移酶(GST)活性。结果:小鼠连续多次灌服虎杖苷后可显著提高其肝脏蛋白质含量,肝指数、肝微粒体细胞色素P450、b5含量和NADPH细胞色素c还原酶活性未见明显变化,肝GST和UDPGT活性显著下降。结论:虎杖苷对小鼠肝微粒体Ⅰ相酶没有明显作用,但抑制II相酶UDPGT和GST。

To observe the effects of polyda.tin on cytochrome P450 and phase Ⅱ transferase. Methods： Polydatin was administered at 60,120,240mg/kg · d in mice for seven days orally. Then total eytochrome P450 and b5 contents, NADPH-eytoehrome P450 reduetase and activities of phase il transferase were detected by speetrophotometer respectively. Results： Compared with the control group, polydatin increased protein content of liver significantly, while didnt affect the hver index, total P450 and b5 contents and the activity of NADPH-eytochrome P450 reduetase（ P 〉0.05 ）. And activities of GST and UDPGT in liver mierosome were decreased significantly by polydatin （P 〈 0. 01 ）, Conclusion： Polydatin has no obvious influence on phase I transferase contents, while it has strong inhibition effects on activities of GST and UDPGT in the mice liver.