注射用新鱼腥草素钠Beagle犬体内药代动力学研究

The investigate of pharmacokinetics of sodium new houttuyfonate injection in beagle dogs

目的：建立犬血浆中新鱼腥草素钠浓度测定的方法，观察注射用新鱼腥草素钠在Beagle犬体内的药代动力学。方法：采用高效液相色谱法进行新鱼腥草素钠的血药浓度测定。色谱柱：辛基硅烷键合硅胶柱（250mm×4，6mm，5μm）；流动相：甲醇-水-四丁基氢氧化铵（70：30：0．3）；检测波长：286nm，流速：1．0ml／min。用DAS新药开发权威统计软件（Ver1．0）计算药代动力学参数，并进行统计学分析。结果：方法线性范围为0.128～25．575mg／L，精密度好，回收率高。新鱼腥草素钠在犬体内的达峰时间约5.0min，平均峰浓度为4.982mg／L，消除半衰期为8.67min。结论：新鱼腥草素钠经静脉注射吸收速度较快。

Objective： To establish the determining method of sodium new houttuyfonate in plasma and study the pharmacokinefies of sodium new houttuyfonate in beagle dogs. Methods： The HPLC was employed. The chromatographic conditions used in this study were of octasilicane bond silica gel column （250mm ×4.6mm,5μm） as analytical column, methanol - water - ammonium hydroxide with 4 butyls（70： 30： 0. 3 ） as mobile phase and at the flow - rate of 1.0ml/min. The UV detection was set at 286nm. The pharmacokinetic parameters were calculated by DAS（ ver1. 0） program and compared by statistic analysis. Results： The calibration curve was linear in blood plasma over the concentration range of 0. 128 - 25. 575mg/L （ r = 0. 9992 ）, well precision and high recoveries. The parameters were Tmax = 5.0min, Cmax = 4. 982mg/L,T1/2 = 8.67min. Conclusion： The analytical method established in this study is sensitive and exact, and can be used to measure the pharmacokinetics of sodium new houttuyfonate in beagle dog. The sodium new houttuyfonate can metabolize quickly following intravenous injection.