灯盏细辛注射液中总咖啡酸酯在大鼠体内的药代动力学研究

The pharmacokinetic study of caffeic acid esters after iv Erigeron Injection in rats

目的：研究灯盏细辛注射液中总咖啡酸酯的大鼠体内过程。方法：采用甲醇沉淀法制备血液样品，并用uv法测定。结果：浓度在20～2μg/ml范围内线性关系良好，相关系数r=0．995；样品的回收率在94％～100．8％，日内RSD和日间RSD均小于5％。总咖啡酸酯在大鼠体内的过程符合二室模型（weight=1／c／c），主要药动学参数为：n=0．1595±0．0658min^-1，β=0．0037±0．0014min^-1，V（c）=18．0198±2．0215（mg／kg）／（mg／m1），T1／2α=4．3456±1．2854min，T1／2β=188．6554±10．2556min，K21=0．0212±0．0046min^-1，K10=0．0275±0．0089min^-1，K12=0．1143±0．0594min^-1，AUC=59．8192±4．1564（mg／m1）·min。结论：灯盏细辛注射液中的总咖啡酯类成分在大鼠体内过程符合静脉注射二室模型。

Objective： To study the pharmacokinetics of caffeic acid esters after iv Erigeron injection （灯盏细辛注射液）. Methods： To prepare plasma samples by the methanol precipitation method, and determine the prepared samples by UV, Results： Linearity was obtained over the range of 2 ～20μg/ml, r = 0.995 ;The method recovery was 94% ～ 100.8% ; Inter-day RSD and intra-day RSD all below 5% , the concentration to time curve of caffeic acid esters fitted to two-compartment model with iv injection. The main pharmacokinetic parameters： α =0. 1595 ± 0.0658min^-1,β =0. 0037 ±0. 0014 min^-1 , V （c） = 18. 0198 ± 2. 0215 （ mg/kg）/（ mg/ml）, T1/2α = 4. 3456 ± 1. 2854 min, T1/2β = 188. 6554 ±10.2556 min,K2, =0. 0212 ±0. 0046 min^-1 ,K,0 =0. 0275 ±0. 0089min^-1 ,K,2 =0. 1143 ±0. 0594min^-1 ,AUC =59. 8192 ±4. 1564 （mg/ml） · min. Conclusion： The pharmacokinetics model of coffeic acid esters is two-compartment model after iv Erigeron injection in Sprague-Dawley rats.