大鼠胚胎NSCs与人胚NSCs的比较

Comparison between Rat Embryo Neural Stem Cells and Human Embryo Neural Stem Cells

目的探讨体外培养的大鼠胚胎神经干细胞(NSCs)及人胚NSCs的生长增殖特点。方法取孕15d的大鼠,采用胰酶消化法获取海马区细胞,以106个/ml的细胞密度接种到无血清NSCs培养基中培养,分离纯化至第5代后,以10%胎牛血清(FBS)和2%多聚赖氨酸诱导分化,免疫细胞化学鉴定。同样方法分离、培养人胚海马区细胞。结果取大鼠胚胎NSCs和人胚NSCs的细胞球及诱导分化后的细胞作免疫细胞化学染色鉴定,细胞分别呈巢蛋白(nestin)、微管蛋白(β-tubulin)、胶质纤维酸性蛋白(GFAP)免疫细胞化学反应阳性。结论大鼠胚胎海马区与人胚海马区均有NSCs存在,离体培养时能分裂增殖,并能被诱导分化,且两者形态相似,但是其生长速度、体积大小有差异。

Objective To observe the characteristics of growth and proliferation of the neural stem cells （NSCs） derived from the hippocampus of rat embryos and human embryos in vitro. Methods The cells were isolated from the hippocampus of rat and human embryos. The cell suspension （at concentration of 106 cells/ml） obtained were cultured in the basic culture medium up to the fifth parental generation. The differentiation of the cells was induced by 10% fetal bovine serum and 2% polylysine. The characters of the cells were identified by immunocytochemical staining. Results The immunocytochemical staining showed that nestin, β-tubulin and glial fibrillary acidic protein were positive in both the human NSCs and rats NSCs. Conclusion There are NSCs in the hippocampuses of rat embryos and human embryos. The NSCs can proliferate by fission and be differetiated into neurons and astrucytes in vitro. The NSCs derived from the hippocampuses of rat and embryos are similar to each other in the morphous, but their growth velocity and volumes are different from each other.