荷叶铁线蕨孢子的不同贮藏时间与培养条件对配子体和孢子体形成的影响

Effects of Spore Storage Time and Culture Conditions on the Formation of Gametophyte and Sporophyte of Adiantum reniforme var.sinense

研究了荷叶铁线蕨（Adiantum reniforme var.sinense）的不同贮藏时间与培养条件对其配子体发育和孢子体形成的影响，结果表明：荷叶铁线蕨孢子为不含叶绿素孢子，在室温下保存5年以上时间依然具有活力，但不同的贮藏时间对配子体和孢子体的形成具有显著的影响。其中，室温贮藏7d的配子体形成的时间为29d，而贮藏5年的为38d；贮藏7d的孢子体形成的时间为96d，贮藏4个月的为105d。在培养室[（25±1）℃恒温和每天2000～25001x12h光照]沙基质中，从孢子播种到形成孢子体时间最短，为87d；沙基质比腐殖土更利于孢子体的形成；配子体密度在不超过3株／cm^2的情况下，密度越高越有利于孢子体的形成。

The effect of different spore storage time and cultivate conditions on the formation of gametophyte and sporophyte in Adiantum reniforme var. sinense was investigated in present study. The results showed that the spores of A. reniforme var. sinense was non-chlorophyllous spores and could remain viable in ambient storage for at least five years, but different storage time has a significant effect on formation time of both gametophytes and sporophytes, with that 7 days ambient storage resulting gametophyte formation in 29 days prolonged five year ambient storage resulting sporophyte formation in 38 days, whereas 7 days ambient storage resulting sporophyte formation in 96 days prolonged four month storage resulting sporophyte formation in 105 days. The culture condition （controlled temperature and light： （25 ± 1 ）℃ and 2000 -2500 lx for 12 h/day） in sand media resulted in a shortest sporophyte formation time, 87 days after sporaphyte was inoculated. The sand medium is more favorable than peat soil for sporophyte formation and high density of gametophytes enhanced the sporsphyte formation.