北京地区人乳头瘤病毒16型感染及其E6／E7基因变异与宫颈病变的相关性研究

Association between buman papillomavirus （HPV） type 16 infection and E6/E7 gene variant and the cervical lesions in Beijing

目的 探讨HPV16感染及其E6／E7基因变异与宫颈病变的相关性。方法 采用导流杂交技术进行HPV感染分型检测，PCR扩增出80份HPV16阳性宫颈病变的E6／E7基因、克隆入pMD18-T载体，双向测序分析基因变异与宫颈病变相关性。结果 HPV16在宫颈病变患者中的检出率最高为33．3％（154/463），与病变程度相关（P〈0．05）。E6／E7基因72份测序成功，DNA序列变异发生率为88．9％（64/72）。氨基酸序列E6-D32E（T96G）和E7-N29S（A86G）位点突变同时伴随存在，D32E／N29S的检出率为38．9％（28／72），与宫颈病变程度相关（P〈0．05）。结论 HPV16是北京地区来源的宫颈病变中最常见的致病型，其D32E／N29S变异与病变程度相关。

Objective To investigate the association between HPV16 infection, E6/E7 variations and the cervical lesions. Methods HPV subtypes were detected by using flow-through hybridization technique, E6/E7 gene was extracted firm cervical lesions in 80 patients with HPV16 infection, PCR amplified, cloned into plasmid pMD18-T-and sequenced. Results HPV 16 was the most common type which accounted for 33.3% （154/463）, the HPV16 infection rates increased with the severity of cervical lesions（ P 〈 0.05）. Totally in 72 eases the complete E6 and E7 regions were successfully sequenced, the DNA mutation rate of E6/E7 was 88.9% （64/72）. A mutation, E6-D32E （T96G） coincided with a specific type of E7 mutation, N29S （A86G）. D32E/N29S mutation rate was 38.9% （28/72）, the detection rate increased with the severity of cervical lesions （ P 〈 0.05）. Conclusion HPV 16 was the most common type in women with cervical lesions in Beijing, D32E/N29S variant associated with the cervical lesions.