超顺磁氧化铁标记神经干细胞移植治疗帕金森病大鼠脑MRI改变的研究

Study the changes of brain MRI following transplantation of SPIO labeled neural stem cells in treatment of Parkinson's disease in rat

目的研究应用超顺磁氧化铁（SPIO）标记神经干细胞（NSCs）移植治疗帕金森病（PD）大鼠脑部MRI的改变。方法从大鼠胚胎脑中分离培养中脑NSCs,用脂质体转染法将SPIO标记NSCs;同时制作大鼠PD模型,SPIO标记的NSCs移植到PD大鼠右侧纹状体区,分别在移植后1周、2周、4周、6周、8周和10周给大鼠行MRI检查,2周、4周、6周、8周、10周时进行旋转行为评分,并与NSCs组和对照组比较。结果脑内移植SPIO标记NSCs的PD大鼠1周后MRI检查显示在移植区呈低信号改变;移植后10周,在T2和T2梯度回波仍可观察到移植区的低信号,同时移植区有混杂信号。NSCs移植大鼠的旋转行为评分在移植后2-10周明显少于对照组（均P〈0.05）。结论SPIO标记的NSCs可以在MRI上显示其在移植大鼠脑部的分布和存活情况,有利于NSCs移植治疗PD后的疗效观察。

Objective To investigate the changes of brain MRI following transplantation of superparamagnetic iron oxide （SPIO） labeled neural stem cells （NSCs） in treatment of Parkinson＇s disease in rats. Methods Mutipotential NSCs from the midbrain of SD rats at embryonic day 14 - 16 were prepared. Labeled NSCs were achieved with the magnetic resonance contrast agent SPIO. Lipofectamine was used as transfection agent to achieve high transfer efficiencies. NSCs and SPIO NSCs were transplanted to the right striatal area of 6-OHDA induced PD rat models. MRI scanning was performed at 1,2, 4, 6, 8 and 10 weeks following transplantation and rotational behavior in response to amphetamine was tested at the same time. Results Hypo-intense changes of MRI examination could be observed in the transplanted area of NSCs labeled by SPIO 1 week after transplantation in PD rats. 10 weeks after transplantation, the hypo-intense signals and mixed signals were also found on T2 weighted and T2＊ gradient echo imaging. Rotational behavior in response to amphetamine showed a significant reduction in PD rats transplanted with NSCs labeled with SPIO compared with control group （all P 〈 0. 05 ）. Conclusion SPIO particles may label effectively NSCs, and MRI detection of SPIO labeled cells is a promising method and novel approach to analysis the NSCs following transplantation to treat PD.