细胞因子IL-6mRNA与IL-10mRNA在早产合并绒毛膜羊膜炎胎盘组织中的表达水平及意义

Expression Levels of IL-6mRNA and IL-10mRNA in Placental Tissues of Chorioamnionitis in Preterm Labor and its Significance

目的:研究白细胞介素-6 mRNA(IL-6 mRNA)和白细胞介素-10 mRNA(IL-10 mRNA)在早产合并绒毛膜羊膜炎孕妇胎盘组织中的表达水平,探讨IL-6及IL-10在妊娠中的作用,以及其在早产合并绒毛膜羊膜炎中的变化及意义。方法:取足月分娩及早产孕妇部分胎盘胎膜组织做病理检查,根据病检结果分为3组:早产感染组20例、早产非感染组20例和足月组20例。采用二步法RT-PCR法测定3组胎盘组织中IL-6 mRNA和IL-10 mRNA的表达。结果:早产感染组胎盘组织中IL-6 mRNA表达水平明显高于早产非感染组和足月组,差异有显著性(P<0.05);而IL-10 mRNA表达水平明显低于另外两组,差异有显著性(P<0.05)。结论:感染可引起细胞因子IL-6 mRNA、IL-10 mRNA在胎盘组织中的表达发生改变,推测IL-6可促进分娩发动,使妊娠提前终止,而IL-10在维持正常妊娠中可能起重要作用。

Objective：To study the change of intedeukin-6 （IL-6）mRNA and intedeukin-10 （IL-10）mRNA expression levels in placental tissues,and to explore their significance in preterm labor with chorioamnionitis. Methods： Placental tissues collected from women with preterm labor and term labor in corresponding period that be divided into three groups according to pathological result of placenta：20 chorioamnionitis infection preterm labors,20 noninfectious preterm labors,and 20 term labors.Then 1L-6mRNA and IL-10mRNA expression in placental tissues in three groups were tested by two-step reverse transcription polymerase chain reaction （ RT-PCR）. Results： IL-6 mRNA expression level was significantly higher in infectious preterm labor than that in noninfectious preterm labor and term labor （ P 〈 0.05）. IL-10 mRNA expression level in infectious preterm labor was significantly lower than that in another two groups （P〈0.05） .Conclusions. Infection may result the change of IL-6 mRNA and IL-10 mRNA expression levels in placental tissues. IL-6 should promote onset of delivery and premature termination of pregnancy. However, IL-10 may play an important role in maintaining normal pregnancy.