中国上海及其周边省份汉族人群XRCC3基因Thr241Met多态与脑胶质瘤遗传易感性关联研究

Association between XRCC3 Thr241Met polymorphism and genetic susceptibility to glioma in Chinese Han population living in Shanghai and surrounding provinces in east China

目的:分析上海及其周边省份汉族人群中脑胶质瘤患者X线交叉互补修复基因3(X-ray repair cross-complementing group 3,XRCC3)Thr241Met单核苷酸多态性(single-nucleotide polymorphism,SNP),探讨其与脑胶质瘤遗传易感性的相关性。方法:应用TaqMan技术检测我国上海及其周边省份(江苏、浙江、安徽等)汉族人群中771例脑胶质瘤患者(病例组)和752例非肿瘤对照受试者(对照组)XRCC3基因Thr241Met多态性;应用统计学方法对TaqMan技术成功检测分型结果进行处理,分析该位点多态性与脑胶质瘤遗传易感性的相关性。结果:1468例受试者(病例组760例,对照组708例)应用Taq- Man技术成功进行了目标基因检测及分型。基因分型成功率为96．4％。统计学分析发现:对照组C和T等位基因频率以及TC、TT基因型频率与病例组无显著差异。经年龄和性别因素校正,TC基因型(P=0．909;OR=0．981;95％CI=0．701～1．371)或TT基因型(P=0．642;OR=0．7;95％CI=0．156～3．146)与CC基因型相比并不增加脑胶质瘤的发生风险。结论:我国上海及其周边省份汉族人群XRCC3基因Thr241Met的变异基因型TC和TT可能不是脑胶质瘤的遗传高危因素。

Objective：To investigate the possible association between Thr241Met polymorphism in the DNA repair gene X- ray repair cross-complementing group 3 （XRCC3） with genetic susceptibility to glioma in a Chinese Han population living in Shanghai and the surrounding provinces in east China. Methods： Genotyping by a TaqMan assay was performed in 771 brain glioma patients living in Shanghai and the surrounding provinces（Jiangsu, Zhejiang, Anhui, etc. ）and in 752 control participants matched in age and gender. The genotyping results of TaqMan assay and the association between Thr241Met polymorphism in the DNA repair gene XRCC3 with genetic susceptibility to glioma were statistically analyzed. Results： Genotypes of 1 468 subjects （760 with brain glioma and 708 were cancer-free control） were successfully performed by TaqMan assay, with the successful rate being 96.4 %. Statistical analysis result showed that gene（C/T） and genotype（C/C, T/C,T/T） frequencies of XRCC3 were not significantly different between the glioma and cancer-free groups. Compared with the CC genotype, the variant TC（P=0. 909; adjusted by age and gender OR=O. 981; 95%CI=0. 701-1. 371） or TT（P=0. 642; adjusted by age and gender OR=0. 7, 95%CI=0. 156-3. 146） genotypes of XRCC3 Thr241Met were associated with a norrstatistically significant increase of glioma risk. Conclusion： The variant TC or TT genotypes of XRCC3 Thr241Met may not be risk factors for brain glioma in Chinese Han population living in Shanghai and the surrounding provinces in east China.