内皮素受体拮抗剂对糖尿病大鼠视网膜VEGF的作用

Inhibitory effect of endothelin receptor antagonist on increased vascular endothelial growth factor level of retina in diabetic rats

目的观察内皮素受体拮抗剂对糖尿病大鼠视网膜血管内皮生长因子(VEGF)升高的抑制作用。方法31只成年雄性Wistar大鼠随机分为正常对照组、糖尿病非治疗组和糖尿病治疗组(予内皮素受体拮抗剂)。分组饲养24周后取材,ELISA法检测视网膜组织内VEGF水平。结果糖尿病治疗组VEGF水平明显低于糖尿病非治疗组组,经统计学分析,具有非常显著性差异(P<0.01)。结论一定浓度的内皮素受体拮抗剂能降低糖尿病大鼠视网膜内VEGF水平,对糖尿病视网膜病变的发生可能有一定的预防作用。

Objective To observe the inhibitory effect of endothelin receptor antagonist on increased vascular endothelial growth factor （VEGF） level of retina in diabetic rats. Methods Male Wistar rats were randomly divided into three groups： normal control group, diabetic group （ diabetes without treatment） and treated group （ diabetes treated with endothelin receptor antagonist）. The rats were killed after 24 weeks. Levels of VEGF in retina of rats were detected by ELISA. Results Levels of VEGF were significantly lower in treated group than diabetic group （P 〈 0.01）. Conclusion Endothelin receptor antagonist can decrease level of VEGF in retina of diabetic rats, and may potentially prevent the development of diabetic retinopathy.