慢性阻塞性肺疾病患者外周血淋巴细胞TIMP-2的表达

Expression of TIMP-2 in peripheral blood lymphocytes in patients with chronic obstructive pulmonary diseases

目的探讨慢性阻塞性肺疾病(COPD)患者外周血淋巴细胞基质金属蛋白酶组织抑制物2(TIMP-2)的表达及其与吸烟的关系。方法选取44例吸烟COPD患者和42例健康吸烟者作为研究对象,应用逆转录-聚合酶链式反应(RT-PCR)检测并比较COPD患者和健康吸烟者外周血淋巴细胞TIMP-2的表达,并与吸烟指数进行相关性分析。结果COPD患者和健康吸烟者TIMP-2 mRNA的表达分别为0.753±0.154和1.170±0.196,前者明显低于后者,两者相比有显著性差异(P<0.05)。吸烟指数与TIMP-2 mRNA呈明显负相关(r=-0.62,P<0.05)。结论TIMP-2 mRNA表达降低,而导致蛋白酶-抗蛋白酶失衡可能与COPD的发生相关,而吸烟是影响TIMP-2 mRNA表达的较为重要的因素。

Objective To explore the expression of tissue inhibitor of metalloproteinases-2 （TIMP-2） in peripheral blood lymphocytes in patients with chronic obstructive pulmonary diseases （COPD） and the association of smoking and TIMP-2 mRNA. Methods The expression of TIMP-2 mRNA in peripheral blood lymphocytes was measured by reverse transcription-polymerase chain reaction （RT-PCR） in 44 patients with COPD and 42 healthy smokers. The correlation analysis was then conducted between TIMP-2 mRNA expression and smoking index. Results The expression of TIMP-2 mRNA was 0. 753 ± 0. 154 and 1. 170 ± 0. 196, respectively, in patients with COPD and healthy smokers（ P 〈0.05）. The expression of TIMP-2 mRNA was negatively correlated with the smoking index （r = -0.62, P 〈 0.05）. Conclusion The lower expression of TIMP-2, which leads to the imbalance between protease and antiprotease, may be associated with the pathogenesis of COPD. Smoking may play an important role in the expression of TIMP-2 mRNA.