精子顶体酶含量、精子畸形率和麦芽凝集素受体含量的临床意义

Clinical significance of quantitation of spermatozoan surface wheat germ agglutinin receptor,the frequency of abnormitic sperm morphology and the sperm acrosin activities

目的研究常规检查精液的精子膜麦芽凝集素受体(WGA)、精子畸形率和精子顶体酶的临床意义。方法收集单纯因输卵管梗阻行体外受精-胚胎移植(IVF-ET)并于促排卵周期获取成熟卵8个以上患者258例,在患者丈夫精液常规检查均正常后,进行精子WGA受体含量、精子畸形率和精子顶体酶含量的检测,并统计患者受精率。结果体外受精率<65%组64例,受精率≥65%组为194例,两组比较,WGA受体含量有显著性差异,顶体酶活性和精子畸形率差异不显著。结论精子膜麦芽凝集素受体的含量、精子畸形率与精子顶体酶均能影响体外受精,但WGA受体含量更能预见体外受精率。

Objective To determine the predictive value of wheat germ agglutinin receptor （WGAR） on human spermatozoa surface, the frequency of abnormitic sperm morphology and human spermatozoa acrosin activities to fertilization rate of IVF-ET. Methods Spermatozoa and oocytes were obtained from both normospermic men and women with oviduct obstruction and came to our center for IVF procedure. Statistics of the quantitation of WGAR on spermatozoa, the frequency of abnormitic sperm morphology, human spermatozoa acrosin activities and the fertilization rate of IVF-ET were made. Results Two groups based on the fertilization rate less than 65% （n = 64）and over 65% （n = 194） were divided, there was significant on the amount of WGAR （P 〈0.01 ） ;there was no significandy difference between the frequency of abnormitic sperm morphology and human spermatozoa acrosin activities （P 〉0. 01 ;P 〈0. 05）. Conelution The fertihzation rate of IVF-ET was affected by the amount of WGAR on spermatozoa, the frequency of abnormitic sperm morphology and human spermatozoa acrosin activities ,while the amunt of WGAR on spermatozoa might be more useful to predict fertilization rates in vitro.