低氧促进hMSCs向多巴胺能神经元分化

Hypoxia enhanced the differentiation of human bone marrow-derived mesenchymal stem cells into dopaminergic neurons

目的观察低氧对体外培养的人骨髓间充质干细胞(hMSCs)向多巴胺能神经元分化的影响。方法采用细胞免疫化学法、流式细胞分析和高效液相色谱—电化学法,检测多巴胺能神经元的数量以及诱导分化后培养基中多巴胺的含量。结果低氧处理后,低氧分化组酪氨酸羟化酶(TH)阳性神经元数量明显增多,为常氧分化组的3倍(P<0.01),并且分化后的神经细胞合成的多巴胺(DA)及其代谢产物高香草酸(HVA)含量也高于常氧分化组(P<0.05)。结论低氧可促进hMSCs向多巴胺能神经元方向分化,这为hMSCs临床应用于治疗帕金森病提供了新的思路。

Objective To investigate the effect of hypoxia on the differentiation of human bone marrow-derived mesenchymal stem cells （hMSCs） into the dopaminerigic neurons. Methods The number of differentiated cells was counted immunocytochemistry and flow cytometry, the content of dopamine and its metabolits were measured by HPLC-ECD. Results TH positive cells differentiated from BME-induced hMSCs under hypoxic condition increased about 3 times as compared with BME-induced hMSCs under normoxic condition. Furthermore, the content of dopamine （DA） and its derivation homovanillic acid （HVA） synthesized by differentiated cells in hypoxia group were significantly higher than that of normoxia group （P 〈0. 01 and P 〈0. 05 respectively）. Conclusion Hypoxia improves the differentiation of human bone marrow-derived mesenchymal stem cells into dopaminergic neurons; this provides a new clue for clinical treatment of Parkison＇ s Disease with hMSCs.