cDNA微阵列技术研究干旱胁迫下柽柳基因的表达

Study on Expression of Genes in Tamarix androssowii under Drought Stress Using cDNA Microarray Technology

运用cDNA微阵列技术研究干旱胁迫下柽柳(Tamarix androssowii)基因的表达。分别将Cy5和Cy3两种荧光染料标记在干旱处理和对照的柽柳cDNA上,并与载有柽柳基因的微阵列进行杂交,通过计算机对芯片进行扫描和分析研究干旱胁迫下基因的表达。共获得了47个下调表达和62个上调表达的基因。Blastx分析表明这些基因按功能可以分为脱水保护、信号传导与调控、活性氧清除、光合作用、代谢、核糖体蛋白、蛋白质的分解与再生等几大类别。同时,发现了一些与干旱胁迫相关的功能未知基因和新基因。从而揭示了柽柳具有活性氧消除、代谢调节、脱水保护、蛋白质降解与再生等抗旱途径,并阐述了干旱胁迫前后柽柳基因的差异表达。

eDNA mieroarray method was employed to study genes expression pattern in Tamarix androssowii under drought stress, eDNAs from T. androssowii treated with drought stress and control group were labeled with two fluorescence dyestuff Cy5 and Cy3, and hybridize with eDNA mieroarray containing T. androssowii genes. Gene expression under drought stress was studied through scanning and analysis of gene chip by computer. Totally 47 down-regulated genes and 62 up-regulated genes were obtained. Blastx analysis showed that the function of these genes could be grouped into the categorizations such as dehydration protection, signal transduetion and regulation, active oxygen scavenging, photosynthesis, metabolism,ribosomal protein, and protein breakdown and recycling. At the same time, some function un- known genes and new genes related to drought stress were also found. This research revealed some important pathways of drought-resistance in T. androssowii, such as active oxygen scavenging, regulation of metabolism, dehydration protection and protein breakdown and recycling. In addition, the differential ex- pression of genes in T. androssowii before and after drought stress were also elucidated.