摘要
选用花楸茎节为外植体进行组培腋芽增殖途径研究,结果表明:利于花楸生长的最适宜基本培养基为Ms培养基;芽诱导培养基为1/2MS+6-BA 2.5 mg·L^-1 +2.4-D0.5 mg·L^-1;增殖培养基为MS+6-BA(1.600~2.100)mg·L^-1+NAA(0.140~0.230)mg·L^-1,平均繁殖系数达到13倍以上;继代壮苗培养的最佳培养基为MS+6-BAO.2 mg·L^-1+IBA0.2mg·L^-1;在1/2MS+IBA0.3 mg·L^-1培养基上,平均生根数为5.96,生根率达90.20%.将生根后的组培苗移栽至腐殖土:泥炭土:河沙(比例为3∶2∶1)的基质中,成活率达95.55%.
In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA (2. 5 mg·L^-1 ) and 2. 4- D(0.5mg·L^-1); the optimum medium for bud proliferation was MS + 6-BA(1.600 - 2. 100) mg·L^-1 +NAA(0. 140 -0.230)mg·L^-1, 13 shoots per explant could be obtained at average;the shoots grew strongly on MS +6-BA0. 2mg·L^-1 + IBA0. 2 mg·L^-1; And 1/2MS + IBA0. 3mg·L^-1 was the best medium for shoot rooting,with the rooting rate at 90. 20% ,and the average root number of 5.96; Plantlets survival rate reached 95.55% when transplanted on the mixture( humus 3 :peat soil 2:sand 1 ).
出处
《植物研究》
CAS
CSCD
北大核心
2007年第2期224-228,共5页
Bulletin of Botanical Research
基金
大庆地区抗旱耐盐绿化树种资源的选择
引种及产业化繁殖技术研究(大庆市科学技术计划项目)
关键词
花楸腋芽
组织培养
快速繁殖
Sorbus pohuashanensis axillary bud
tissue culture
rapid propagation