花楸腋芽增殖途径快繁技术研究

The Rapid Propagation Technology of Sorbus pohuashanensis by Axillary Bud Regeneration Way

选用花楸茎节为外植体进行组培腋芽增殖途径研究,结果表明：利于花楸生长的最适宜基本培养基为Ms培养基;芽诱导培养基为1/2MS＋6-BA 2.5 mg·L^-1 ＋2.4-D0.5 mg·L^-1;增殖培养基为MS＋6-BA（1.600～2.100）mg·L^-1＋NAA（0.140～0.230）mg·L^-1,平均繁殖系数达到13倍以上;继代壮苗培养的最佳培养基为MS＋6-BAO.2 mg·L^-1＋IBA0.2mg·L^-1;在1/2MS＋IBA0.3 mg·L^-1培养基上,平均生根数为5.96,生根率达90.20%.将生根后的组培苗移栽至腐殖土：泥炭土：河沙（比例为3∶2∶1）的基质中,成活率达95.55%.

In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA （2. 5 mg·L^-1 ） and 2. 4- D（0.5mg·L^-1）; the optimum medium for bud proliferation was MS ＋ 6-BA（1.600 - 2. 100） mg·L^-1 ＋NAA（0. 140 -0.230）mg·L^-1, 13 shoots per explant could be obtained at average;the shoots grew strongly on MS ＋6-BA0. 2mg·L^-1 ＋ IBA0. 2 mg·L^-1; And 1/2MS ＋ IBA0. 3mg·L^-1 was the best medium for shoot rooting,with the rooting rate at 90. 20% ,and the average root number of 5.96; Plantlets survival rate reached 95.55% when transplanted on the mixture（ humus 3 ：peat soil 2：sand 1 ）.