TfR1 mRNA和IRP1 mRNA在不同铁状态孕妇胎盘中的表达

The expression of TfR1 mRNA and IRP1 mRNA in the placenta from different maternal iron status

目的测定转铁蛋白受体1（TfR1）和铁调节蛋白1（IRP1）在不同铁状态足月孕妇胎盘中的mRNA表达水平，探讨胎盘铁转运及调控机制。方法采用RT—PCR方法测定正常组、铁缺乏（ID）组及缺铁性贫血（IDA）组足月孕妇胎盘中TfR1和IRP1的mRNA相对表达水平。结果①正常组TfR1 mRNA为0．4813±0．1891，ID组为0．6647±0．2788，IDA组为0．9767±0．2858，IDA组与正常组和ID组相比差异均有统计学意义（分别t=0．002，P〈0．01；t=0．028，P〈0．05），而ID组与正常组之间差异无统计学意义（t=0．117，P〉0．05）。②正常组IRP1 mRNA为0．2616±0．0785，ID组为0．3696±0．1801，IDA组为0．3971±0．0902，各组间差异无统计学意义（F=1．845，P=0．179）。结论①胎盘TfR1 mRNA随孕妇缺铁程度加重而增加，表明IRP1参与胎盘TfR1 mRNA的调节。②不同铁状态孕妇胎盘组织中IRP1 mRNA无量的显著变化，明确了IRP1发挥调节作用不是通过自身量的变化，但TfR1 mRNA表达水平的变化说明IRP1在胎盘铁代谢中发挥调节作用。

Objective To investigate the mRNA expression of transferrin receptorl （TfR1）and iron regulatory proteinl （ IRP1 ） in the full term placenta from different maternal iron status,and explore the mechanism of placental iron transport and regulation. Methods The mRNA level of TfR1 and IRP1 in full term placentae was detected by reverse transcription polymerase chain reaction （RT-PCR） in normal group （ N ）, iron deficiency group （ID）and iron deficiency anemia group （IDA）. Results （1） The expression of TfR1 mRNA in N group was 0. 4813 ±0. 1891, in ID group was 0. 6647 ±0. 2788,and in IDA group was 0.9767 ± 0.2858. There was significant difference between IDA group and N group or ID group （t=0. 002, P〈0.01 or t =0. 028,P 〈0.05）, and was no difference between ID group and N group（t =0. 117,P 〉 0.05）. （2）The expression of IRP1 mRNA in N group was 0. 2616±0. 0785,in ID group was 0. 3696±0. 1801,and in IDA group was 0.3971±0.0902 and was no difference among the three groups （F= 1.845, P =0. 179）. Conclusions The expression of TfR1 mRNA is increased when maternal iron deficiency progressed while there is no change in the expression of IRP1 mRNA in the placentae of TfRlmRNA indicated that IRP1 takes part in the regulation of placenta iron transport.