肺癌组织中Cyr61基因表达及临床意义

Expression of CYR61 in human lung cancer tissues and its clinical significance

目的:研究人肺癌组织中Cyr61基因表达的临床意义。方法:采用实时荧光定量-PCR技术和免疫组织化学染色法对60名肺癌患者的肺癌组织和癌旁肺组织的Cyr61 mRNA和蛋白表达水平进行测量。结果:80%(48/60)肺癌患者的癌组织中Cyr61表达水平低于其配对的癌旁肺组织中的水平,与免疫组织化学染色法测定的结果相一致。统计分析显示,两组间差异有统计学意义(2.742±4.165vs4.933±3.349,t=-5.112,P=0.000)。肿瘤恶性程度分级、淋巴结转移、病理学类型、吸烟状况及家族史等临床参数均可影响癌组织中的Cyr61的表达水平(P<0.05)。结论:Cyr61对肺癌的进展起重要作用,因此Cyr61表达的蛋白产物可作为临床诊断的重要指标。

Objective： To investigate the biological functions of the Cyr61 gene in human lung carcinomas. Methods：Tbe Cyt61 expression levels in the cancerous and peri-cancerous tissues of 60 lung cancer patients were measured by real-time polymerase chain reaction （PCR）. Results： The expression ofCyr61 gene was down-regulated in 80% （48/60） primary lung cancer patients compared with the matched pericancerous tissues. The result was consistent with the immunohistochemical staining. Statistical analysis revealed that the difference between expression of cancerous tissues and the matched peri-cancerous tissues was significant [ （2. 742±4. 165 ） vs （4. 933 ±3. 349）, t = -5. 112, P =0.000]. Furthermore, the clinical variables including tumor grade, tumor metastasis, tumor pathological classification, smoking, and family history influenced the level of Cyr61 expression （P 〈 0.05 ）. Conclusion：Cyr61 may play a key role in the progression of lung carcinoma and its protein might serve as an important target for the therapeutic intervention in clinic.