准分子激光和紫外线照射对胰岛免疫原性的影响

The Influences of Irradiation of Excimer Laser and an Ultraviolet Beam On Immunogenecity of Pancreatic Islet

新生大鼠胰岛样细胞团经准分子激光和中波紫外线照射预处理后,其免疫原性明显降低,激光组尤为显著,形态学检查示胰岛细胞分泌颗粒和亚细胞结构保持完整无损。胰岛素分泌功能与对照组无明显差异。经STZ引致的糖尿病大鼠中胰岛异体移植,结果表明激光组受体大鼠糖尿病缓解时间较紫外线组与对照组明显延长。

After the pretreatment of neonatal rat islet-like cell clusters (ICCs) with irradiation of an excimer laser and an ultraviolet beam (UVB), the immunogenecity of islet was significantly reduced, especially in the excimer laser group. Morphological examination of the pretreated islets revealed that the secretory granules and subcellular structures remained intact, and the insulin secretion function of the pretreated islets showed no significant difference with that of the control group. The islet allotransplantation in STZinduced diabetes rats indicated that the diabetes remission period of the rats of the excimer laser group was significantly longer than that of the UVB group and the control group.