黄芩苷降解细菌内毒素的定量分析测定

Study on Anti-endotoxin of Baicalin

目的比较不同浓度黄芩苷降解内毒素的作用。方法采用连续倍比稀释的方法,依次选取6.250、3.125、1.562、0.781μg/mL黄芩苷溶液,分别与1EU/mL内毒素工作标准品混合,分别于(37±1)℃恒温水浴15、30、60min,应用鲎实验检测反应液中内毒素含量。结果6.250μg/mL黄芩苷对1EU/mL的细菌内毒素的降解作用最强,15min内完全降解。3.125、1.562μg/mL黄芩苷分别在30min和60min内完全降解。在同一时间段内,4种不同浓度的黄芩苷降解内毒素的作用依次减弱。结论黄芩苷降解内毒素的作用有浓度依赖性和时间依赖性,随黄芩苷浓度升高,降解内毒素作用逐渐增强;随作用时间延长,黄芩苷降解内毒素作用增强。该结果提示黄芩苷有较好的降解细菌内毒素的作用。

Objective To study the anti-endotoxin of different concentration baicalin. Methods 6.250 μg/mL, 3.125 μg/mL, 1.562 μg/mL and 0.781 μg/mL baicalin solutions were mixed with 1 EU/mL endotoxin, respectively. The mixtures were put into water of （37±1） ℃ for 15 min, 30 min and 60 min. The degrading effects were determined by using limulus amebocyte lysate test （LAL test）. Results 1）The degrading effect of 6.250 μg/mL, 3.125 μg/mL and 1.562 μg/mL baicalin solution on 1 EU/mL endotoxin was degraded completely in 15 min, 30 min and 60 min, respectively. 2）The degrading effect of 3.125 μg/mL, 1.562 μg/mL and 0.781 μg/mL baicalin solution on 1 EU/mL endotoxin after these mixtures had been incubated for 15 min. Endotoxin values were （0.155 5±0.002 8） EU/mL, （0.212 1±0.004 9） EU/mL, （0.355 9±0.013 9） EU/mL, respectively. These differences among them were statistically significant（P〈0.01）. 3）The degrading effect of 1.562 μg/mL and 0.781 μg/mL baicalin solution on 1 EU/mL endotoxin after these mixtures had been incubated for 30 min. Endotoxin values were （0.164 0±0.002 5） EU/mL and （0.209 4± 0.004 4） EU/mL, respectively. These differences between them were statistically significant（P〈0.01）. Conclusion The action of anti-endotoxin of baicalin is dose-dependent and time-dependent. The results show that baicalin has the stronger anti-endotoxin effect.