Survivin基因在顺铂诱导Tca8113舌鳞癌细胞凋亡中的作用

Role of Survivin Gene on the Apoptosis of Tca8113 Cells Induced by Cisplatin

目的体外观察顺铂(DDP)诱导Tca8113舌鳞癌细胞株凋亡的作用,同时检测在此过程中凋亡蛋白抑制因子Survivin mRNA表达和蛋白表达的变化。方法将人Tca8113舌鳞癌细胞株进行传代培养,MTT法检测顺铂不同浓度和不同时间对Tca8113舌鳞癌细胞生长的抑制作用,通过RT-PCR检测凋亡过程中Survivin基因mRNA的表达,免疫细胞化学观察Survivin基因的蛋白表达,以及流式细胞术观察顺铂诱导各组Tca8113细胞的凋亡率。结果顺铂可明显抑制Tca8113舌鳞癌细胞的增殖,其增殖抑制率呈浓度和时间依赖性,细胞凋亡率也呈同样的趋势,最高可达34.1%。1.0μg/mL DDP处理Tca8113舌鳞癌细胞,Survivin mRNA和蛋白表达水平随时间的增加而降低,在24h达到最低,随后又升高。结论抗凋亡蛋白Survivin在Tca8113舌鳞癌细胞高表达,顺铂可有效诱导Tca8113舌鳞癌细胞凋亡,Survivin mRNA表达在化疗早期随作用时间的延长而降低,Survivin基因的抑制在顺铂诱导的Tca8113舌鳞癌细胞的细胞凋亡中起着重要的作用。

Objective To observe the induction of apoptosis of cisplatin （DDP） to oral squamous cell carcinoma cell line （Tca8113） in vitro and study the role of Survivin on the apoptosis of Tca8113 cells induced by cisplatin. Methods The inhibitory effects of different doses of DDP on Tca8113 cells were assayed with MTT test. Apeptosis was determined by flow cytometry. The expression of Survivin was detected by RT-PCR and immunocytochemistry. Results Cisplatin obviously inhibited Tca8113 cells growth in a dose and time dependent manner. The apoptotic index showed the similar trend. Survivin gene expression was decreased with increasing of time and reached the lowest level at 24 hours after DDP treatment, then increased after that time. Conclusion Cisplatin gene can effectively induce apeptosis in Tca8113 cells and the inhibition of Survivin gene expression may play a critical role on Tca8113 cell apoptosis induced by cisplatin.