阻遏蛋白β-TrCP在小鼠牙胚不同发育时期的表达与意义

β-transduction Repeat Containing Protein Expressed in Tooth Germs and Ameloblast and Odontoblast of Different Stage of Tooth Development

目的观察Sonic hedgehog(shh)信号转导通路的阻遏蛋白-βTrCP在小鼠牙胚发育不同时期的表达情况,探讨-βTrCP在晚期牙胚发育中的作用与意义。方法取不同发育时期的鼠胚、乳鼠鼠头标本,用标记生物素链亲和素LsAB法观察β-TrCP蛋白在不同发育时期牙胚组织的表达情况。结果β-TrCP在胚胎10.5、13.5、14.5、16.5、18.5 d的小鼠牙胚上皮层与间充质层,以及出生后0、3、6 d的小鼠成釉细胞与成牙本质细胞胞浆呈特征性表达。结论-βTrCP在正常发育小鼠牙胚及成釉细胞与成牙本质细胞特征性表达,提示-βTrCP在牙胚发育中维持/限定shh信号通路的正常信号转导具有重要意义。

Objective The Sonic hedgehog signalling peptide has been demonstrated to play important roles in the growth and patterning of the tooth development. This study aims on whether the antagonist β-transduction repeat containing protein of Sonic hedgehog signal transduction expressed in tooth germs ameloblast and odontoblast or not. Methods The mouse embryo heads of different developmental stages of E10.5, E13.5, E14.5, E16.5, E18.5 and P0, P3, P6 after birth were acquired fixed with 4% paraformaldehyde for 48 hours, embeded with Paraffin and examined using LsAB （labelled streptavidin-biotin） method to observe the β-TrCP expression pattern in tooth germs, ameloblast and edontoblast. Results It was demonstrated that β-TrCP expressed in oral epithelium, tooth bud, mesenchymal cell cytoplasm of ameloblast and edontoblast of different stage of tooth development. Conclusion β-TrCP expressed from early stage to later stage of murine tooth development. And these findings provide the evidence of antagonist regulatory pathways for shh in teeth development.