银屑病患者骨髓造血细胞体外增殖活性研究

Proliferative Activity of Bone Marrow-derived Hematopoietic Cells of Psoriatic Patients In Vitro

目的进一步了解银屑病患者骨髓造血细胞是否有异常。方法采用密度梯度离心法分离银屑病患者及正常对照组骨髓单一核细胞,然后在含有甲基纤维素的半固体细胞因子培养体系中进行体外高增殖潜能集落形成细胞(HPP-CFC)、粒-巨噬细胞系集落形成单位(CFU-GM)及红系集落形成单位(CFU-E)集落形成实验,培养一定时期后计数集落。结果与正常对照比较,银屑病患者的HPP-CFC及CFU-GM的集落形成数显著减少,而CFU-E形成的集落数无统计学意义。结论银屑病患者骨髓造血细胞体外增殖能力明显降低,这种异常的造血细胞可能在银屑病的免疫发病机制中发挥一定作用。

Objective To address the question of whether the hematopoietic cells involve in the immunopathogenesis of psoriasis. Methods Marrow mononuclear cells were isolated from bone marrow of psoriatic patients and normal controls by density gradient centrifugalization, and colony forming assays of high proliferative potential colony forming cells （HPP-CFCs）, granulocyte -macrophage colony-forming units （CFUs-GM） and erythroid colony- forming units （CFUs-E） were performed in vitro in methylcellulose semi - solid culture medium in the presence of cytokines. Results For the colony forming assays of bone marrow mononuclear cells derived from psoriatic patients in comparison to the normal controls, the colony numbers of HPP-CFC and CFU-GM were significantly reduced,however the colony formation of CFU-E did not present a decrease or increase of the expansion efficiency. Conclusion The in vitro proliferative ability of hematopoietic cells derived from bone marrow with psoriasis is abnormal, and the abnormal hematopoiesis maybe involve in the pathngenesis of psoriasis.