实验性精索静脉曲张大鼠血清及睾内睾酮的变化

Effects of Experimental Varicocele on the Testosterone Level in the Serum and Testis of Rats

目的:研究精索静脉曲张(VC)大鼠模型对血清T和睾丸内T的影响。方法:通过部分结扎左肾静脉建立SD大鼠VC模型20只;假手术组SD大鼠20只为对照组。模型建立后4、8周取静脉血,并取部分睾丸组织匀浆提取上清液,放免法测定血清及睾丸内T浓度。结果:血清T:实验组4、8周与同期对照组相比有下降趋势,但没有统计学意义。双侧睾丸内T水平:实验组4周与对照组4周相比下降,但无统计学意义(P>0.05);实验组8周与对照组8周相比下降,具有统计学意义(P<0.01)。结论:在8周内实验性大鼠VC并没有引起血清T的降低,但可以导致双侧睾丸内T降低。

Objective： To study the effects of experimental varicocele （VC） on the serum testosterone （T） and intratesticular testosterone in adolescent rats. Methods ： A VC rat model was established by partial ligation of the left kidney vein in 20 SD rats, and another 20 were included in a sham operation group as controls. At 4 and 8 weeks, the concentrations of the serum T and intratesticular T were measured by radioimmunoassay （RIA）. The testis tissues were homogenized and the extract liquid taken for RIA. Results： Compared with the controls, the level of serum T declined at 4 and 8 weeks in the VC group, but not significantly （ P 〉 0.05 ）, so was that of bilateral intratesticular T at 4 weeks, and with statistical significance at 8 （ P 〈 0.01 ）. Conclusion ： Within 8 weeks, experimental VC could reduce the level of bilateral intratesticular T, but not that of serum T. Varicocele could damage Leydig cells.