实验犬急性胰腺炎时MIF、ICAM-1的动态变化及意义

Significance of dynamic change of serum MIF and ICAM-I levels in canine experimental acute pancreatitis.

目的研究犬实验性急性胰腺炎时血清巨噬细胞移行抑制因子（MIF）、细胞间黏附分子-1（ICAM-1）的动态变化及在AP分型判断中的应用价值。方法将犬随机分为对照组、急性水肿性胰腺炎（AEP）和急性坏死性胰腺炎（ANP）3组，每组7条。静脉滴注雨蛙素（5ug·kg^-1·h^-1）诱导AEP模型，胰管内注射5％牛磺胆酸钠（0．1mg／kg体重）诱导ANP模型。采用ELISA法检测MIF、ICAM-1和血淀粉酶含量。胰腺组织常规病理学检查。结果对照组血MIF、ICAM-1和血淀粉酶含量分别为（6．23±0．21）ng／ml、（2．4±0．8）ng／ml和（256．3±95．8）U／L，AEP组造模后3h时的值分别为（18．564±1.30）ng／ml、（56．4±15．1）ng／ml和（1420．0±175．5）U／L，显著高于对照组（P〈0．01）。且随时间延长而逐渐增高。ANP组在造模后3h的值分别为（25．72±4．23）ng／ml、（195．2±27．9）ng／ml和（1686．3±180．8）U／L，显著高于对照组，也显著高于AEP组（P〈0．01）。胰腺的病理学改变分别符合水肿性和坏死性胰腺炎的改变。结论血MIF、ICAM-1含量在AP早期即升高，且与胰腺病变的严重程度相关，可作为判断SAP还是MAP的一种辅助指标。

Objective To study dynamic change of serum macrophage migration inhibitory factor （MIF） and intercellular adhesion molecule-1 （ICAM-1）MIF levels in canine experiment acute pancreatitis and the significance in distinguishing acute necrotizing pancreatitis （ANP） from acute edema pancreatitis（AEP）. Methods Twenty-one canines were equally randomized into three groups： the normal group, AEP group and ANP group. AEP model was induced by intravenous injection of cerulean （5 ug · kg^-1 · h^-1）. ANP model was induced by retrograde injection of 5% sodium taurocholate （0. 1 mg/kg body weight）. Serum MIF, ICAM-1 and amylase were detected by ELISA. Pancreatic tissues were pathologically examined. Results Serum levels of MIF, ICAM-1 and amylase were （6. 23 ± 0. 21）ng/ml, （2. 4 ±0. 8）ng/ml, and （256. 3 ± 95.8）U/L, respectively, in the normal group. Serum levels of MIF, ICAM-1 and amylase were （18. 56 ± 1.30）ng/ml, （56. 4 ± 15. 1）g/ml, and （1 420. 0 ± 175.5）U/L, respectively, in AEP group at 3 hours, and significantly higher than that in the normal group （P 〈 0. 05）. Serum levels of MIF, ICAM-1 and amylase were （25. 72 ±4. 23） ng/ml, （195. 2 ± 27. 9）ng/ml, and （1 686. 3± 180. 8）U/L, respectively, in ANP group, with significantly higher than that in both the normal group and AEP group（P 〈 0. 01）. Histological changes of the pancreas in each group conformed to the pathological characterization of normal, AEP and ANP. Conclusions Serum levels of MIF and ICAM-1 elevated in early of AP, and were related to the severity of the condition, and they can be used as an accessory marker for differential diagnosis of SAP and MAP.