RANTES、MIP-1α趋化因子促人肝癌细胞侵袭运动的机制研究

Mechanism of RANTES and MIP-1α promoting invasion and migration of helmtoma carcinoma cells

目的探讨RANTES、MIP-1α趋化因子促人肝癌细胞侵袭运动的机制。方法使用流式细胞仪和激光共聚焦显微镜观察RANTES、MIP-1α刺激后人肝癌细胞聚合型肌动蛋白（F-actin）聚合的变化。采用明胶酶谱法分析RANTES、MIP-1α对人肝癌细胞基质金属蛋白酶分泌及活性的影响。结果RAN-TES、MIP-1α可诱导人肝癌细胞内F-actin的聚合，并刺激细胞突起和伪足形成。明胶酶谱显示RANTES、MIP-1α刺激后人肝癌细胞基质金属蛋白酶MMP-2、MMPO等活性显著增加，降解基质能力增强。结论RANTES、MIP-1α趋化因子可以诱导F-actin聚合促进人肝癌细胞定向运动，并通过增加基质金属蛋白酶活性提高人肝癌细胞侵袭能力。

Objective To investigate mechanism of chemokines including RANTES and MIP-1α promoting invasion and migration of hepatoma carcinoma cells （HCC）. Methods The polymerization of HCC F-actin stimulated by RANTES and MIP-1α was evaluated by flow cytometry and confocal microscope. Gelatin zymography assay was used to observe effect of RANTES and MIP-1α on secretion and activity of matrix metalloproteinase of HCC. Results Both RANTES and MIP-1α could prominently promote polymerization of F-actin and formation of cell process and pseudopodia of HCC. Gelatin zymography assays showed that the activities and degeneration potential of MMP-2 and MMP-9 in HCC were increased following stimulation with RANTES and MIP-1α. Conclusions Both RANTES and MIP-1α can promote migration of HCC by inducing polymerization of F-actin and increase invasive potential of HCC by enhancing activity of MMPs.