加味补阳还五汤对8Hz 130dB次声暴露下小鼠大脑过氧化水平及超微结构的影响

An experimental study on the effect of oxidation resistance and ultrastructure of Jia Wei Bu Yang Huan Wu Decoction after infrasound exposure(8Hz,130dB)

目的:观察加味补阳还五汤对8Hz 130dB次声暴露下小鼠大脑过氧化水平及超微结构的影响。方法:40只BALB/c小鼠分为空白对照组、次声对照组、次声加用药组(根据用药剂量的不同又分高、中、低剂量3个亚组),处理14d后测试其超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-PX)活性、丙二醛(MDA)含量,以及在电镜下观察其大脑皮质超微结构的变化。结果:次声对照组的SOD活力与正常对照组相比有明显降低,各用药组中的SOD活力较次声对照组有明显升高,其中以高剂量组最为明显;次声对照组的GSH-PX活力与正常对照组相比有明显升高,各用药组中的GSH-PX活力较次声对照组也有明显升高;次声对照组的MDA含量与正常对照组相比有明显升高,高、中剂量用药组中的MDA含量较次声对照组有明显降低。透射电镜观察次声对照组的大脑皮质的神经元周围可见小角质细胞水肿、增生;线粒体畸形明显,呈分叉、三角形,并可见空泡化;高尔基体中度扩张;毛细血管周围间隙明显,髓鞘板层排列紊乱,脂褐素增多明显。在用药组中上述损伤有所减轻。结论:8Hz 130dB次声暴露14d(2h/d)可引发小鼠大脑皮质的脂质过氧化,给小鼠造成一定的损伤。加味补阳还五汤可以通过提高小鼠体内GSH-PX和SOD的活性来清除体内的自由基,使MDA含量降低,减轻次声对机体作用后的不良反应。

Objective To study the effects of Jia Wei Bu Yang Huan Wu Decoction on oxidation resistance after ultra-structure of mice exposed to 130dB infrasound （8Hz）. Method： Forty BALB/c mice were divided into blankcontrol group, infrasound-control group, infrasound and drug-treated group （it was divided into 3 sub-groups according to drug dose）. SOD and GSH-PX activities and MDA contents were tested and ultrastrueture of pallium were observed 14 days afterward. Result： Compared with blank group, SOD activity decreased （P〈0.05）,and MDA content increased （P〈0.01） in simple infrasound group; Compared with simple infrasound group, SOD activity increased（P〈0.01）, MDA content decreased（P〈0.01） in infrasound and drug-treated group. Conclusion： 8Hz 130dB infrasound could damage learning and memory abilities causing by lipid peroxidation reaction in mice＇s cortex. Jia Wei Bu Yang Huan Wu Decoction could eliminate free radicals by enhancing the activity of GSH- PX and SOD, decreasing the content of MDA, and alleviating side effect of the brain caused by infrasound.