SLE患者外周血白细胞B7-H1分子的表达

B7-H1 expression on peripheral blood mononuclear cells from patients with systemic lupus erythematosus

目的探讨B7-H1分子在系统性红斑狼疮（SLE）患者外周血白细胞的表达。方法应用荧光抗体染色技术．经流式细胞仪检测30例SLE患者和20例正常人对照者外周血白细胞表面B7-H1的自然表达水平．同时收集SLE患者的实验室检查指标，并用SLE疾病活动指数（SLEDAI）来判定患者疾病活动程度。结果活动期SLE组单核细胞、淋巴细胞和粒细胞上B7-H1表达水平均明显高于正常人对照组（P〈0．01）；SLE患者单核细胞和淋巴细胞B7-H1表达水平与SLEDAI呈正相关（r1=0．42，只〈0．05；r2=0．52，P2〈0．05）；抗dsDNA抗体阳性患者组淋巴细胞表面B7-H1分子数较抗dsDNA抗体阴性组明显增高（t=3．20，P〈0．01）。治疗后SLE患者外周血单核细胞、淋巴细胞和粒细胞B7-H1表达均明显下降，t值分别为10．00、6．75和4.33，P〈0．01。结论活动期SLE患者外周血白细胞B7-H1表达增加．且与SLEDAI成正相关，B7-H1可能与SLE的发病机制有关。

Objective To investigate the expression of B7-H1 on peripheral blood mononuclear cells （PBMCs） fi＇om patients with systemic lupus erythematosus （SLE）. Methods Immunofluorescent staining and flow cytometry assay were used to measure the expression level of B7-H1 on PBMCs from 30 SLE patients and 20 healthy controls. Results of laboratory examination of patients were collected. SLE disease activity index （ SLEDAI ） was used to evaluate the disease severity. Results The expression levels of B7-H1 on monocytes, lymphocytes and granulocytes was markedly higher in active SLE group than those in control group （all P 〈 0.01 ）, respectively. The expression of B7-H1 on monocytes and lymphocytes was positively correlated with SLEDAI of patients （ r1 = 0.42, P1 〈 0.05; r2 = 0.52, P2 〈 0.05 ）, respectively. On the other hand, B7-H1 expression on lymphocytes was significantly higher in patients with positive anti-dsDNA antibody than that in patients with negative anti-dsDNA antibody （ t = 3.20, P 〈 0.01 ）. After three weeks＇ treatment with glucocorticoid, the level of B7-H1 was significantly decreased on the monocytes, lymphocytes and granulocytes of patients in comparison with that before treatment （ t = 10.00, 6.75, 4.33, respectively, all P〈 0.01 ）. Conclusions The expression of B7-H1 on PBMCs increased in active SLE, and there was positive correlation between B7-H 1 expression and SLEDAI, which suggests that B7-H 1 may be relevant to the pathogenesis of SLE.