豹皮樟总黄酮对大鼠酒精性脂肪性肝炎的防治作用及部分机制

The protective and therapeutic effects of total flavonoids of litsea coreana level on alcoholic steatohepatitis and its mechanisms

目的探讨豹皮樟总黄酮(TFLC)对大鼠酒精性脂肪性肝炎的防治作用及部分机制。方法以酒精联合脂肪乳剂灌胃,每天2次,连续6周,制备大鼠酒精性脂肪性肝炎模型。给药组同时灌服相应药物。于实验的第6周末采血,检测血清丙氨酸氨基转移酶(ALT)、天门冬氨基转移酶(AST)、γ-谷氨酰基转移酶(γ-GT)、碱性磷酸酶(ALP)、总胆固醇(TC)、甘油三酯(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)及肝脏TG、TC、SOD、MDA、GSH-PX,并行肝脏病理学检查。结果TFLC(200、400mg/kg)组能明显降低酒精性脂肪性肝炎大鼠血清AST、ALT、ALP、γ-GT水平;降低血清和肝匀浆中TC、TG、MDA含量,增强SOD和GSH-PX活性;同时病理组织学显示TFLC能明显改善酒精性脂肪性肝炎大鼠的肝细胞脂肪变性。结论TFLC对大鼠酒精性脂肪性肝炎具有较好的防治作用。其作用机制与抗氧化和调节脂质代谢有关。

Objective To explore the protective and therapeutic effects of total flavonoids of litsea coreana level （TFLC） on alcoholic steatohepatitis and its mechanisms. Methods The rats of the model group were fed with ethanol and fat emulsion twice a day for 6 weeks. At the same time, the rats of TFLC group and tiopronin group were administrated with relative dose of medicine. By the end of the 6th week, all rats were sacrificed. The serum ALT, AST, ALP, γ-GT, TG, TC, SOD, MDA, GSH-PX and hepatic TG, TC, SOD, MDA, GSH-PX were measured respectively. Hepatic histopathology was observed by microscope. Results Serum levels of ALT, AST, ALP, γ-GT decreased significantly in administration of TFLC（200,400 mg/kg）, both serum and hepatic levels of TG,TC ,MDA markedly reduced , meanwhile the activity of SOD and GSH-PX were enhanced. The histopathological analysis suggested that TFLC could significantly ameliorate fatty degeneration of hepatocytes. Conclusion TFLC has positively protective and therapeutic effects on alcoholic steatohepatitis. The pharmacological mechanisms are related with antioxidation and adapting blood lipid component.