瑞金医院烧伤中心甲氧西林耐药金黄色葡萄球菌同源性分析

Analysis of methlcillin resistant Staphylococcus aureus in burn ward of Ruijin Hospital

目的 从分子生物学水平分析甲氧西林耐药金黄色葡萄球菌（MRSA）在上海瑞金医院烧伤中心院内感染的流行状况。方法 用WHONET5统计软件分析2003-2005年瑞金医院烧伤中心MRSA的分布状况；用随机扩增多态DNA（RAPD）技术对本中心住院患者临床标本中分离的17株MRSA进行同源性分析。结果 分离的17株MRSA为同一RAPD型（引物为ERIC2、RAPD7）。结论 本中心多年来持续存在和流行的MRSA为同一个型别，应成为瑞金医院烧伤中心抗感染治疗和院内感染控制的重点菌株。

Objective To analyze the epidemiology of methicillin resistant Staphylococcus aureus （MRSA） in molecular level in burn centre of Shanghai Ruijin hospital, Methods The vicissitude of Staphylococcus aureus in the burn centre from 2003 to 2005 was analyzed with software WHONETS. Multiprimer random amplified polymorphic DNA（RAPD） was used to analyze the homology of 17 MRSA strains. Results RAPD analysis （primer ERIC2 and RAPD7） showed that all 17 MRSA strains were identical （Burn-A type）. Conclusion MRSA with same RAPD type is prevalent in our burn centre for many years, so emphasis should be laid on the anti-infection therapy and its cross infection control.