许旺细胞与小肠黏膜下层体外复合培养的形态学研究

Morphological study of co-culture of Schwann cells with small intestinal submucosa in vitro

目的观察体外培养的许旺细胞在小肠黏膜下层(SIS)表面的黏附生长状况,探讨SIS与许旺细胞(SCs)的生物相容性。方法体外分离培养SD乳鼠的许旺细胞,接种于制备好的SIS进行复合培养,不同时间段通过相差显微镜、组织学、扫描电镜和透射电镜观察SCs在SIS上的黏附、生长和增殖情况。结果相差显微镜下见3～5 d后SIS边缘SCs生长良好;组织学观察见5 d时许旺细胞与SIS表面黏附紧密;扫描电镜观察见SIS表面SCs增殖黏附良好,胞体突起显著,呈端对端的相互连接或排列成束,细胞表面可见有蛋白颗粒分泌。透射电镜观察见SIS表面许旺细胞生长状态良好,细胞紧密贴附生长于SIS表面;许旺细胞与SIS交界部见细胞底部形成一些突起与SIS接触。结论SCs能够在SIS表面良好地黏附生长,SIS作为支架材料,与SCs具有良好的生物相容性。

Objective To observe adhesion and growth of Schwann cells （SCs） on small intestinal submucosa（SIS） and study the biocompatibility of SIS with SCs. Methods The SCs of SD neonate rat were isolated and cultured in vitro, then were seeded on prepared SIS. At different times, the adhesion, growth and proliferation of SCs on SIS were observed by phase contrast microscope, histological examination, scanning electron microscope and transmission electron microscope. Results By phase contrast microscope, SCs grew well on the edge of SIS after 3 and 5 days. SCs adhered tightly on the surface of SIS after 5 days through histological examination. By scanning electron microscope, on the surface of SIS, SCs grew and adhered actively, prominence of cells body were obvious. They connected end-to-end with each other or arranged in clusters. Protein granules were secreted on cells surface. By transmission electron microscope, SCs grew in good condition and adhered tightly on the surface of SIS. At the interface of SCs and SIS, prominence was seen to contact with SIS in the bottom of cell body. Conclusion SCs are able to adhere and grow well on the surface of SIS. As a scaffold, SIS has good biocompatibility with SCs.