超敏反应辅助蛋白基因(hrap)转化番茄的研究

Studies on Transformation of hrap Gene into Tomato

将可以诱发植物对病原菌抗性的甜椒hrap基因置于35S启动子的驱动下,经农杆菌介导引入番茄。再生植株经过含卡那霉素的培养基的选择,后经PCR检测和Southern杂交鉴定含有目标基因。Northern杂交检测其表达后,初步的抗菌检验显示其提高了对青枯病原菌的抵抗力。

Sweet pepper hrap gene under the control of CaMV 35S promoter has been transformed into tomato via Agrobacterium tumefaciens mediated procedure. Plantlets were regenerated on MS medium with 3.0 mg · L^-1 6-BA, 0. 2 mg· L^ - 1 IAA, and 50 mg · L^-1 kanamycin. The transgenic plants had been identified by PCR procedure and Southern blot, and their expression was showed by Northern hybridization. A preliminary assay with leaf disc infected by tomato pathogen Ralstonia solanacearum Smith showed that transgenic plants had higher resistance to the pathogen than wild-type tomato.